DUTCH Adrenal Hormone Stress Test


The adrenal hormone stress test provides detailed information on how adrenal and sex hormones are being produced and metabolized in the body.

Markers Included on This Test

  • Daily free cortisol (the amount available and circulating in the body),
  • Metabolized cortisol (the amount of cortisol the adrenals are producing or clearing),
  • Hormone metabolites (estrogen, progesterone, testosterone, and DHEA),
  • Melatonin,
  • Phase I (hydroxylation) and phase II (methylation) of estrogen metabolism, and
  • DHEA and testosterone clearance.

Collection Instructions:

Detailed instructions are included in your kit. Please follow them exactly as stated. Please specifically note the instructions in relation to:

  • The timing of collection. Pre-menopausal women should collect between days 19 – 22 of a 28-day cycle. Men, peri-menopausal, and menopausal women can collect on any day.
  • The timing of taking hormones whilst doing the test.
  • Avoid caffeine and large amounts of fluids, restricting fluids somewhat 2 hours prior to each collection.
  • Don’t forget to label the back of each sample with your name and circle which one it is: waking, 2 hours after waking, dinner, bedtime. Very important not to forget this.

Here are also some videos to help you:



Samples can be collected in any order, but the recommended pattern is usually the easiest to follow.



  • Test Type: Dried urine (urine is collected onto filter paper strips at 4 specific times throughout the day)
  • Test Cost: Within the USA US$259, Outside the USA US$275 which is paid directly to the lab.
  • Turnaround Time: 5-10 business days
  • Instruction Sheet: Download Test Instructions
  • Sample ReportDownload Sample Report